postmortem als brain tissue from motor and occipital cortex  (Johns Hopkins HealthCare)

Journal: Cell reports

Article Title: Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis

doi: 10.1016/j.celrep.2023.113160

Figure Lengend Snippet: (A and B) Immunohistochemistry of VCP and SOD1 in postmortem motor (affected) (A) and occipital (unaffected) (B) cortex from an ALS patient (patient #1-JHU74) carrying the A4V mutation in the SOD1 gene. The pictures on the right column represent the magnified regions within the yellow dashed squares. Scale bar, 20 μm. (C) Quantification of SOD1 and VCP intensities in MAP2 + neurons within the motor or occipital cortex. Motor cortex, n = 17 neurons; occipital cortex, n = 16 neurons. Unpaired t test (two-tailed), SOD1 p < 0.0001; VCP p < 0.0001.

Article Snippet: Patient 1 (JHU74), postmortem ALS brain tissue from motor and occipital cortex , Johns Hopkins University , SOD1 +/A4V , Male,47 (age of death).

Techniques: Immunohistochemistry, Mutagenesis, Two Tailed Test

Journal: Cell reports

Article Title: Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis

doi: 10.1016/j.celrep.2023.113160

Figure Lengend Snippet:

Article Snippet: Patient 1 (JHU74), postmortem ALS brain tissue from motor and occipital cortex , Johns Hopkins University , SOD1 +/A4V , Male,47 (age of death).

Techniques: Cell Culture, Sterility, Recombinant, Multiplex sample analysis, Western Blot, Stripping, Protease Inhibitor, Plasmid Preparation, Activity Assay, Magnetic Beads, Ligation, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Virus, Software